Major Collision Investigation Unit detectives continue to investigate a collision between a truck and school bus in Bacchus Marsh early on Wednesday morning (Sep. 21).
The collision on the Western Highway, near the intersection of Condons Lane, caused the bus to roll down an embankment about 3.15am.
Two teenage girls sustained serious injuries and were airlifted to a Melbourne hospital. The bus driver, a man in his 50s, sustained minor injuries and was transported to hospital
The truck driver, a man in his 50s, was taken to hospital by road with serious injuries.
All other occupants of the bus, a further 25 students, and four adults, were transported to hospital for observation.
Speed limits had been reduced on the road following a minor collision between two vehicles the previous evening.
Major Collision Investigation Unit detectives continue to investigate the exact circumstances surrounding the incident.